cd86 rabbit pab Search Results


cd86  (Sino Biological)
92
Sino Biological cd86
Cd86, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd86+rabbit+pab/CD86+%2F+ETC-1+%2F+B7-2+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/bio_rxiv__2024__07__30__605730-247-23-25
Average 92 stars, based on 1 article reviews
cd86 - by Bioz Stars, 2026-10
92/100 stars
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rabbit anti cd86 antibody  (ABclonal Biotechnology)
95
ABclonal Biotechnology rabbit anti cd86 antibody
Figure 1 AR-derived Tfhs in a DC co-culture system promote DC maturation. (A) Gating strategy and flow plot for Tfhs sorting according to Materials and Methods. (B) A schematic of Tfhs/DCs Transwell co-culture system. (C) Relative mRNA expression levels of CD80 or <t>CD86</t> in treated or untreated DCs are expressed as mean ± standard error from at least three independent experiments. *** P < 0.001; **** P < 0.0001. (D) Flow cytometry results of DC cell surface expression of the co-stimulatory molecules CD80, CD86 and MHCII in control (no treatment), WT mice-derived Tfhs, AR mice-derived Tfhs, or LPS treated group. Flow cytometry results demonstrate that the ratio of CD80, CD86 and MHC II of DCs was significantly increased in the AR-derived Tfhs/DCs co-culture group. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.
Rabbit Anti Cd86 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd86+rabbit+pab/CD86+Rabbit+pAb/10__2147_slash_jir__s365217-41-34-51
Average 95 stars, based on 1 article reviews
rabbit anti cd86 antibody - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
cd86 rabbit pab  (ABclonal)
N/A
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cd86 antibody, rabbit pab, antigen affinity purified  (Sino Biological)
N/A
Produced in rabbits immunized with purified, recombinant Human CD86 (rh CD86; Catalog#10699-H08H; NP_008820.2; Met1-His239). CD86 specific IgG was purified by Human CD86 affinity chromatography.
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cd86/b7-2 antibody, rabbit pab, antigen affinity purified  (Sino Biological)
N/A
Produced in rabbits immunized with purified, recombinant Mouse CD86/B7-2 (rM CD86/B7-2; Catalog#50068-M08H; NP_062261.3; Met1-Glu245). CD86/B7-2 specific IgG was purified by Mouse CD86/B7-2 affinity chromatography.
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cd86 / b7-2 antibody, rabbit pab, antigen affinity purified  (Sino Biological)
N/A
Produced in rabbits immunized with purified, recombinant Rat CD86 / B7-2 (rR CD86 / B7-2; Catalog#80025-R08H; NP_064466.1; Met 1-Ile 250). CD86 / B7-2 specific IgG was purified by Rat CD86 / B7-2 affinity chromatography.
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cd86 b7 2 antibody rabbit pab  (Sino Biological)
N/A
Produced in rabbits immunized with purified recombinant Rat CD86 B7 2 Catalog 80025 R08H NP 064466 1 Met1 Ile250 Total IgG was purified by Protein A affinity chromatography
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cd86 / etc-1 / b7-2 antibody, rabbit pab  (Sino Biological)
N/A
Produced in rabbits immunized with purified, recombinant Mouse CD86 extracellular domain (rM CD86; Catalog#50068-M08H; NP_062261.3; Met 1-Glu 245). Total IgG was purified by Protein A affinity chromatography.
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cd86 / b7-2 / b70 antibody, rabbit pab, antigen affinity purified  (Sino Biological)
N/A
Produced in rabbits immunized with purified, recombinant Human CD86 / B7-2 (rh CD86; Catalog#10699-H08H; NP_008820.2; Met 1-His 239). CD86 specific IgG was purified by human CD86 affinity chromatography .
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Image Search Results


Figure 1 AR-derived Tfhs in a DC co-culture system promote DC maturation. (A) Gating strategy and flow plot for Tfhs sorting according to Materials and Methods. (B) A schematic of Tfhs/DCs Transwell co-culture system. (C) Relative mRNA expression levels of CD80 or CD86 in treated or untreated DCs are expressed as mean ± standard error from at least three independent experiments. *** P < 0.001; **** P < 0.0001. (D) Flow cytometry results of DC cell surface expression of the co-stimulatory molecules CD80, CD86 and MHCII in control (no treatment), WT mice-derived Tfhs, AR mice-derived Tfhs, or LPS treated group. Flow cytometry results demonstrate that the ratio of CD80, CD86 and MHC II of DCs was significantly increased in the AR-derived Tfhs/DCs co-culture group. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 1 AR-derived Tfhs in a DC co-culture system promote DC maturation. (A) Gating strategy and flow plot for Tfhs sorting according to Materials and Methods. (B) A schematic of Tfhs/DCs Transwell co-culture system. (C) Relative mRNA expression levels of CD80 or CD86 in treated or untreated DCs are expressed as mean ± standard error from at least three independent experiments. *** P < 0.001; **** P < 0.0001. (D) Flow cytometry results of DC cell surface expression of the co-stimulatory molecules CD80, CD86 and MHCII in control (no treatment), WT mice-derived Tfhs, AR mice-derived Tfhs, or LPS treated group. Flow cytometry results demonstrate that the ratio of CD80, CD86 and MHC II of DCs was significantly increased in the AR-derived Tfhs/DCs co-culture group. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Co-Culture Assay, Expressing, Flow Cytometry, Control

Figure 2 Expression of CD80 and CD86 of DCs under four different conditions. Immunofluorescence staining was performed to determine the expression level of CD80 and CD86 on DC cell surface. Scale bar at the bottom right stands for 50 μm. Data shown are representative of 3 independent experiments. Green represents CD80 staining, red CD86 staining, and blue DAPI staining for cell nuclei.

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 2 Expression of CD80 and CD86 of DCs under four different conditions. Immunofluorescence staining was performed to determine the expression level of CD80 and CD86 on DC cell surface. Scale bar at the bottom right stands for 50 μm. Data shown are representative of 3 independent experiments. Green represents CD80 staining, red CD86 staining, and blue DAPI staining for cell nuclei.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Expressing, Immunofluorescence, Staining

Figure 3 Tfhs secrete exosomes and AR-derived Tfh exosomes promote DC maturation. (A) Morphology of exosomes isolated from WTor AR mice by scanning electron microscopy. Scale bar represents 100 nm. (B) Comparison of relative mRNA expression level (mean ± standard error of at least three independent experiments) of CD80 and CD86 in the DCs treated with control (no treatment), WT mice-derived Tfh exosome, AR mice-derived Tfh exosome, LPS, or WT mice-derived Tfh exosomes with the addition of GW4869. *** P < 0.001; **** P < 0.0001. (C) Flow cytometry results of DC cell surface expression of the co-stimulatory molecules CD80, CD86 and MHCII in control (no treatment), WT mice-derived Tfhs exosome, AR mice-derived Tfhs exosome group, LPS, or WT mice-derived Tfh exosomes with the addition of GW4869 group. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 3 Tfhs secrete exosomes and AR-derived Tfh exosomes promote DC maturation. (A) Morphology of exosomes isolated from WTor AR mice by scanning electron microscopy. Scale bar represents 100 nm. (B) Comparison of relative mRNA expression level (mean ± standard error of at least three independent experiments) of CD80 and CD86 in the DCs treated with control (no treatment), WT mice-derived Tfh exosome, AR mice-derived Tfh exosome, LPS, or WT mice-derived Tfh exosomes with the addition of GW4869. *** P < 0.001; **** P < 0.0001. (C) Flow cytometry results of DC cell surface expression of the co-stimulatory molecules CD80, CD86 and MHCII in control (no treatment), WT mice-derived Tfhs exosome, AR mice-derived Tfhs exosome group, LPS, or WT mice-derived Tfh exosomes with the addition of GW4869 group. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Isolation, Electron Microscopy, Comparison, Expressing, Control, Flow Cytometry

Figure 4 Expression of CD80 and CD86 on DC cells under five different conditions. Sections of DCs were stained Immunofluorescence dyes for CD80 (green) and CD86 (red). DAPI was used to stain nuclei (blue). Representative images are shown from at least three independent experiments. Scale bar at the bottom right stands for 50 μm.

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 4 Expression of CD80 and CD86 on DC cells under five different conditions. Sections of DCs were stained Immunofluorescence dyes for CD80 (green) and CD86 (red). DAPI was used to stain nuclei (blue). Representative images are shown from at least three independent experiments. Scale bar at the bottom right stands for 50 μm.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Expressing, Staining, Immunofluorescence

Figure 5 miR-142-5p in AR mouse-derived exosomes regulated DC maturation. (A) Heat map of differentially expressed miRNAs. The heat map was divided into two columns (left: AR; right: Control). Up- or down-regulation of the miRNAs was represented with red or green color, respectively. A specific miRNA, miR-142b, was down- regulated in AR, as indicated with a yellow arrow. (B) The expression level of miR-142-5p in exosomes of Tfhs from AR vs control mice. (C) Detection of the expression level of CD80 and CD86 in different groups with overexpression or inhibition of miR-142-5p. *** P < 0.001; **** P < 0.0001. (D) Flow cytometry analysis of co-stimulatory molecules CD80, CD86 and MHCII on surface of DCs transfected with miR-142-5p mimics or inhibitors. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 5 miR-142-5p in AR mouse-derived exosomes regulated DC maturation. (A) Heat map of differentially expressed miRNAs. The heat map was divided into two columns (left: AR; right: Control). Up- or down-regulation of the miRNAs was represented with red or green color, respectively. A specific miRNA, miR-142b, was down- regulated in AR, as indicated with a yellow arrow. (B) The expression level of miR-142-5p in exosomes of Tfhs from AR vs control mice. (C) Detection of the expression level of CD80 and CD86 in different groups with overexpression or inhibition of miR-142-5p. *** P < 0.001; **** P < 0.0001. (D) Flow cytometry analysis of co-stimulatory molecules CD80, CD86 and MHCII on surface of DCs transfected with miR-142-5p mimics or inhibitors. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Control, Expressing, Over Expression, Inhibition, Flow Cytometry, Transfection

Figure 6 miR-142-5p targets CDK5 to mediate the regulation of DC maturation by Tfhs derived from AR mice. (A) The potential miR-142-5p target sites in CDK5 transcript. (B) Levels of relative luciferase activity in HEK293T cells following transfection with PGL-3-control-WT-miR-142-5p mimics, PGL-3-CDK5-WT-miR-142-5p mimics and PGL-3-CDK5-MT-miR-142-5p mimics respectively (1 μg). The relative luciferase activity refers to the ratio of firefly luminescence/renilla luminescence. (C) Relative expression of miR-142-5p after CDK5 knockdown. (D) CDK5 expression level under overexpression or inhibition of miR-142-5p by RT-qPCR. The values shown are mean ± SD of three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. (E) CDK5 expression level under overexpression or inhibition of miR-142-5p by Western Blot. (F) Flow cytometry assesses the CD80, CD86 and MHCII expression on the surface of DCs by inhibiting CDK5. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 6 miR-142-5p targets CDK5 to mediate the regulation of DC maturation by Tfhs derived from AR mice. (A) The potential miR-142-5p target sites in CDK5 transcript. (B) Levels of relative luciferase activity in HEK293T cells following transfection with PGL-3-control-WT-miR-142-5p mimics, PGL-3-CDK5-WT-miR-142-5p mimics and PGL-3-CDK5-MT-miR-142-5p mimics respectively (1 μg). The relative luciferase activity refers to the ratio of firefly luminescence/renilla luminescence. (C) Relative expression of miR-142-5p after CDK5 knockdown. (D) CDK5 expression level under overexpression or inhibition of miR-142-5p by RT-qPCR. The values shown are mean ± SD of three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. (E) CDK5 expression level under overexpression or inhibition of miR-142-5p by Western Blot. (F) Flow cytometry assesses the CD80, CD86 and MHCII expression on the surface of DCs by inhibiting CDK5. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Luciferase, Activity Assay, Transfection, Control, Expressing, Knockdown, Over Expression, Inhibition, Quantitative RT-PCR, Western Blot, Flow Cytometry

Figure 7 Tfh-derived exosomes promote DC cell maturation through miR-142-5p/CDK5/STAT3 pathway. (A) Relative expression of miR-142-5p and CDK5 in DCs upon LPS stimulation at different time points. The values shown are mean ± SD of three independent experiments. ** P < 0.01; **** P < 0.0001. (B) CDK5 expression level changed with prolonged LPS stimulation. (C) Detection of STAT3 phosphorylation in overexpression and inhibition of miR-142-5p. (D) Detection of STAT3 phosphorylation in inhibition of miR-142-5p with or without si-CDK5. (E) Expression of CD80 and CD86 on DCs with STAT activator or STAT inhibitor and LPS stimulation for different times. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 7 Tfh-derived exosomes promote DC cell maturation through miR-142-5p/CDK5/STAT3 pathway. (A) Relative expression of miR-142-5p and CDK5 in DCs upon LPS stimulation at different time points. The values shown are mean ± SD of three independent experiments. ** P < 0.01; **** P < 0.0001. (B) CDK5 expression level changed with prolonged LPS stimulation. (C) Detection of STAT3 phosphorylation in overexpression and inhibition of miR-142-5p. (D) Detection of STAT3 phosphorylation in inhibition of miR-142-5p with or without si-CDK5. (E) Expression of CD80 and CD86 on DCs with STAT activator or STAT inhibitor and LPS stimulation for different times. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Expressing, Phospho-proteomics, Over Expression, Inhibition

Figure 8 Results from an in vivo experiment showing the effect of AR-derived Tfh exosomes on occurrence of AR. (A) Hematoxylin and eosin staining of nasal mucosal from different group of mice. Scale bars at the bottom right of each image represent 50 µm. The area of mucosal thickening and inflammation appeared as dark blue area in the image, with one area indicated with a yellow arrow. (B) The relative mRNA expression of CD80 and CD86 in mouse nasal mucosa of various groups. The data shown are mean ± standard error of at least 3 independent experiments. (C) Flow cytometry results of the co-stimulatory molecules CD80, CD86 and MHCII in DCs. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments. (D) Inflammatory factors including IFN-γ, IgE, IL-13 and IL-17 are studied in the serum of mice of various group. ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 8 Results from an in vivo experiment showing the effect of AR-derived Tfh exosomes on occurrence of AR. (A) Hematoxylin and eosin staining of nasal mucosal from different group of mice. Scale bars at the bottom right of each image represent 50 µm. The area of mucosal thickening and inflammation appeared as dark blue area in the image, with one area indicated with a yellow arrow. (B) The relative mRNA expression of CD80 and CD86 in mouse nasal mucosa of various groups. The data shown are mean ± standard error of at least 3 independent experiments. (C) Flow cytometry results of the co-stimulatory molecules CD80, CD86 and MHCII in DCs. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments. (D) Inflammatory factors including IFN-γ, IgE, IL-13 and IL-17 are studied in the serum of mice of various group. ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: In Vivo, Derivative Assay, Staining, Expressing, Flow Cytometry